mouse insulin elisa kit  (Cusabio)

Journal: Nature Communications

Article Title: The E3 ligase tripartite motif 7 drives the progression of non-alcoholic fatty liver disease by targeting DUSP10 degradation in male mice

doi: 10.1038/s41467-025-65415-6

Figure Lengend Snippet: a Schematic illustration of the AAV8-mediated DUSP10 downregulation in the liver of HFD-fed Trim7-HKO mice. b , c Immunoblotting analysis ( b ) and quantitative ( c ) analysis of DUSP10 expression in liver tissues from the above AAV8-injected mice ( n = 4; P values determined by 2 tailed t test). d – h Liver weight ( d ), liver weight/body weight ratio ( e ), fasting blood glucose levels ( f ), fasting insulin levels ( g ), and HOMA-IR ( h ) in the above AAV8-injected mice after 14 weeks of HFD feeding ( n = 8; P values determined by 2 tailed t test). i , j The IPGTT ( i ) and IPITT ( j ) were conducted on the above AAV8-injected mice at 12 weeks and 13 weeks of HFD feeding, respectively. Subsequently, the areas under the curve (AUC) for each test were calculated ( n = 8; P values determined by 2 tailed t test). k Immunoblotting analysis of the indicated proteins expression in liver tissues from the above AAV8-injected mice after 14 weeks of HFD feeding ( n = 4). l – n Liver PAS staining images ( l ), liver H&E and Oil red O staining images ( m ), and quantitative analysis of Oil Red O-positive areas in liver tissues ( n = 5; P values determined by 2 tailed t test). o ELISA analysis of the serum levels of TNFα, IL-6, and IL-10 in the above AAV8-injected mice after 14 weeks of HFD feeding ( n = 8; P values determined by 2 tailed t test). p Immunofluorescence staining images for F4/80 in liver tissues from the above AAV8-injected mice ( n = 6). q Immunoblotting analysis of the indicated protein expression in liver tissues from the above AAV8-injected mice ( n = 4). Data are presented as mean ± SD. Source data are provided as a Source Data file.

Article Snippet: The insulin (KE10089), TNFα (KE10002), IL-6 (KE10007) and IL-10 (KE10008) ELISA kits were purchased from Proteintech (Wuhan, China).

Techniques: Western Blot, Expressing, Injection, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Frontiers in Endocrinology

Article Title: Effect of rhPTH(1-34) and alendronate on the treatment of type 2 diabetic bone disease

doi: 10.3389/fendo.2025.1657481

Figure Lengend Snippet: rhPTH had a better effect on improving bone turnover level in DM mice. (A) Representative images of tartrate-resistant acid phosphatase (TRACP) staining of femur (n = 5 per group). Scale bar, 100 μm. (B) Percentage of TRACP positive area calculated by Image J program (n = 5 per group). (C, D) Serum procollagen type I intact N-terminal (P1NP) and C-terminal cross-linking telopeptide of type I collagen (CTX) concentrations determined by ELISA (n = 5 per group). CON, control mice; DM, type 2 diabetes mice; rhPTH, recombinant human parathyroid hormone; ALN, alendronate. Data are represented as means ± SD. * p <0.05, ** p <0.01, *** p <0.001.

Article Snippet: Serum insulin (nIU/mL) were detected using enzyme-linked immunosorbent assay (ELISA) kits from CUSABIO (Cusabio Biotech Cat# CSB-E05071m, RRID: AB_2916335, coefficient of variation of intra- and inter-assay <10%).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control, Recombinant